Gilles Crambert

 

Research projects:

 

Our main interest is focused on the mechanisms that allow kidney to adapt its functions to the physiological environment. More precisely, these last years, we have investigated how the kidney maintain potassium balance 1/ under dietary K+ restriction 2/ during gestation and 3/ during activity-rest alternance (circadian rhythm).

These studies highlighted the fact that a renal ion transporter named H,K-ATPase type 2 (HKA2 also known as the “non-gastric” or the “colonic” H,K-ATPase) has to be stimulated in order to preserve plasma K+ parameters in all three situations mentioned above (Figure 1). 

 

 Moreover, we described a new renal regulatory pathway involving progesterone, HKA2 and renal K+ retention. We used methods involving molecular biology techniques, cell culture, normal and genetically-modified mouse models and metabolic analysis. 

       

 

 

 

 Figure 1: Summary of the physiological contexts in which H,K-ATPase type 2 is involved.

 


 

 

Team:

Gilles Crambert: CR1 CNRS

Christne Lamouroux : Engineer

 

 

 

Representative publications:

 

2018

Renal potassium handling in carriers of the Gly40Ser mutation of the glucagon receptor suggests a role for glucagon in potassium homeostasis. Bankir L, Barbato A, Russo O, Crambert G, Iacone R, Bouby N, Perna L, Strazzullo P. Physiol Rep. 2018 Apr;6(8):e13661. PMID: 29671960

 

2017

Versatility of NaCl transport mechanisms in the cortical collecting duct. Edwards A, Crambert G. Am J Physiol Renal Physiol. 2017 Dec 1;313(6):F1254-F1263. PMID: 28877883

 

2016

 

H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction. Walter C, Tanfous MB, Igoudjil K, Salhi A, Escher G, Crambert G. Pflugers Arch. 2016 Oct;468(10):1673-83. PMID : 27562425

 

The renal cortical collecting duct: a secreting epithelium? Morla L, Doucet A, Lamouroux C, Crambert G, Edwards A. J Physiol. 2016 Oct 15;594(20):5991-6008. PMID: 27412964

 

Glucagon actions on the kidney revisited: possible role in potassium homeostasis. Bankir L, Bouby N, Blondeau B, Crambert G. Am J Physiol Renal Physiol. 2016 Aug 1;311(2):F469-86. PMID: 27194722

 

New insights into sodium transport regulation in the distal nephron: Role of G-protein coupled receptors. Morla L, Edwards A, Crambert G. World J Biol Chem. 2016 Feb 26;7(1):44-63. PMID: 26981195

 

2014

 

Epithelial sodium channel abundance is decreased by an unfolded protein response induced by hyperosmolality. G. Crambert, T. Ernandez, C. Lamouroux, I. Roth, E. Dizin, P.Y. Martin, E. Feraille AND U. Hasler. Physiol Rep. 2014 Nov 20;2(11). PMID: 25413317

 

H,K-ATPase type 2: relevance for renal physiology and beyond. G. CRAMBERT. AJP Renal. (2014) 306,F693-F700. PMID:24431203

 

2013

A link between fertility and K+ homeostasis: role of the renal H,K-ATPase type 2. A. Salhi, C. Lamouroux,  N.B. Pestov, N.N. Modyanov, A. Doucet and G. Crambert. Pflugers Arch. (2013) 465, 1149-1158. PMID: 23440459

 

Renal proteinase activated receptor 2: A new actor in the control of blood pressure and plasma potassium level. L. Morla, G. Brideau, M. Fila, G. Crambert, L. Cheval, P. Houillier, S. Ramakrishnan, M. Imbert-Teboul and A. Doucet. J. Biol. Chem. (2013) 288, 10124-10131. PMID:  23430254

 

2012

Circadian expression of H,K-ATPase type 2 contributes to the stability of plasma K+ levels. A. Salhi, G. Centeno, D. Firsov and G. Crambert. FASEB J. (2012) 26, 2859-2867. PMID: 22459151

 

Expression profile of nuclear receptors along male mouse nephron segments reveals a link between ERRβ and thick ascending limb function. H. Krid, A. Dorison A. Salhi, L. Cheval and G. Crambert. Plos ONE (2012) e34223. PMC: 3311618

 

Regulation of pendrin by cAMP: possible involvement in b adrenergic-dependent NaCl retention. A. Azroyan, L. Morla, G. Crambert, K. Laghmani, S. Ramakrishnan, A. Edwards and A. Doucet.  Am. J. Physiol. (Renal Physiol.) (2012) 302, F1180-1187. PMID: 22262479

 

2011

Chronic potassium depletion increases adrenal progesterone production which is necessary for efficient renal retention of potassium. B. Elabida, A. Edwards, A. Salhi, A. Azroyan, H. Fodstad, P. Meneton, A. Doucet, M. Bloch-Faure and G. Crambert. Kidney Int. (2011) 80, 256-262. PMID: 21326170

 

Edwards. Regulation of pendrin by pH: dependence on glycosylation. A. Azroyan, K. Laghmani, G. Crambert, D. Mordasini, A. Doucet and A. Biochem. J. (2011) 434, 61-72. PMID: 21073444

 

2010

Membrane progestin receptors mPR : beyond the controversy, can we move forward ? A. Salhi, J. Lemale, N. Paris, M. Bloch-Faure, G. Crambert. Biomol. Concepts. (2010) 1, 41-47. ARTICLE

 

 2009

Mapping of sex hormone receptors and their modulators along the nephron of male and female mice. A. Grimont, M. Bloch-Faure, B. El Abida, G. Crambert.  FEBS Lett. (2009) 583, 1644-1648. PMID: 19401201

 

2008

Membrane progestin receptor a and g in renal epithelium. J. Lemale, M. Bloch-Faure, A. Grimont, B. Elabida, M. Imbert-Teboul and G. Crambert. BBA-Molec. Cell Res. (2008), 1783, 2234-2240. PMID: 18722485

 

Proteinase-activated receptor 2 stimulates Na,K-ATPase and sodium reabsorption in native kidney epithelium. L. Morla, G. Crambert, D. Mordasini, G. Favre, A. Doucet, and M. Imbert-Teboul. J. Biol. Chem. (2008), 283, 28020-28028. PMID: 18678869